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　　Mongolian medicine Cha Gan Beng Ga regulated
　　activity of biomarker PGC-1α
　　LI Han-qing 1，2*， XU Jia-yin 3， YU Lu4， XIN Ji-le 1，2， WEI Ji-wen5*
　　（1. State Clinical Trial Institution of New Drugs， International Mongolian Hospital of Inner Mongolia， Hohhot 010065， China；
　　2. Mongolian Medicine Laboratory， Mongolian Medicine Institute of Inner Mongolia， Hohhot 010065， China；
　　3. Mongolian Pharmaceutical Preparation Center， International Mongolian Hospital of Inner Mongolia， Hohhot 010065， China；
　　4. Department of Pharmaceutical Sciences， International Mongolian Hospital of Inner Mongolia， Hohhot 010065， China；
　　5. Chongqing Three Gorges Medical College， Chongqing 404120， China）
　　[Abstract] Objective： To investigate the regulation of Cha Gan Beng Ga on the activity of biomarker PGC-1α in vivo and in vitro， and lay the foundation for studying the efficacy result of Cha Gan Beng Ga on xenograft tumor model and extracting active constituents. Method： ①The coarse powder of Cha Gan Beng Ga was extracted with 70% ethanol solution through heating and refluxing， and finally was used to freeze dry powder. ②50 mg·kg^-1 of freeze-dried power was orally administrated to KM and C57BL/6J mice once daily， lasting for 5 consecutive days； different concentrations of extracted materials was given to non-small cell lung cells A549. ③The expression level of PGC-1α mRNA was quantitatively determined in lung tissue of mice and non-small cell lung cells A549. Result： The expression levels of PGC-1α in lung tissue of different mice strains had an increasing tendency. Furthermore， the expression levels of PGC-1α in non-small cell lung cells A549 also had an increasing tendency， showing dose and time-dependent relationships. Conclusion： Mongolian Medicine Cha Gan Beng Ga could induce the over-expression of PGC-1α mRNA in lung tissue of mice and in non-small cell lung cells A549. The present results will lay foundation for studying the efficacy result of antitumor and active constitutes in future.
　　[Key words]Mongolian medicine； energy metabolism； antitumor； biomarker； PGC-1α
　　doi：10.4268/cjcmm20141733

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